proteasome substrate suc llvy amc  (MedChemExpress)

Journal: Toxicology

Article Title: An adverse outcome pathway-based approach to assess the neurotoxicity by combined exposure to current-use pesticides.

doi: 10.1016/j.tox.2023.153687

Figure Lengend Snippet: Fig. 4. Evaluation of the effects of pesticides using the proteasome activities as endpoints. Cells were exposed to each pesticide (100 µM) or MG-132 (1 µM) as a positive control for 24 h, and then (A) chymotrypsin-like (Suc-LLVY-AMC) and (B) caspase-like (Z-LLE-AMC) proteasome activities were monitored by AMC- conjugated fluorescence substrates. All data are shown as the mean ± SD. * p < 0.05, * * p < 0.01 vs. the control group (one-way ANOVA followed by Dunnett’s post-hoc test).

Article Snippet: The proteasome assay buffer (100 μL) [50 mM HEPES buffer (pH 7.8) containing 10 mM NaCl, 1.5 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 250 mM sucrose, 5 mM DTT and 2 mM ATP] containing 2.5 μM of the fluorogenic proteasome substrate Suc-LLVY-AMC (succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin, #S-280) and Z-LLE-AMC (Z-Leu-Leu-Glu-7-amino-4methylcoumarin, #S-230; Boston Biochem, Cambridge, MA, USA) were added to the lysate sample (25 μL) in a 96-well black plate and incubated at 37 ◦C for 30 min.

Techniques: Pesticides, Positive Control, Fluorescence, Control